gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of Steatohepatitis.
Daria SkuratovskaiaAnastasia DakhnevichEgor ShunkinOlga KhaziakhmatovaValeria ShupletsovaMaria VulfAleksandra KomarElena KirienkovaLarisa S LitvinovaPublished in: Biomedicines (2023)
Obesity is the main cause of metabolic complications. Fatty liver infiltration is a companion of obesity. NAFLD is associated with impaired energy metabolism with an excess of nutrients. Mitochondrial dynamics are important for the regulation of energy balance, which regulates mitochondrial function, apoptosis, and mitophagy. The aim of this study was to investigate the effect of gp130 on the components of mitochondrial dynamics in a cellular model of steatohepatitis. Addition of IL-6/gp130 contributed to an increase in the percentage of live cells and a decrease in the percentage of dead and apoptotic cells. Addition of IL-6/gp130 increased the expression of NF-kB1 gene and mitochondrial dynamics markers (MFN2 and TFAM) in HepG2 with tBHP/Oleic. Addition of IL-6 or gp130 reduced the expression of cytoprotector genes (HSF1 and HSP70) in HepG2 cell cultures with tBHP/Oleic. Increased mitochondrial dynamics gene activity protected against HepG2 cell death in the steatohepatitis model. Trans-signaling resulted in increased TFAM and MAPLC3B, and decreased DNM1L gene expression in HepG2 with tBHP/Oleic.
Keyphrases
- oxidative stress
- cell cycle arrest
- cell death
- induced apoptosis
- gene expression
- pi k akt
- genome wide
- metabolic syndrome
- poor prognosis
- insulin resistance
- weight loss
- signaling pathway
- type diabetes
- copy number
- dna methylation
- weight gain
- heavy metals
- genome wide identification
- single cell
- stem cells
- bone marrow
- high fat diet induced
- risk assessment
- immune response
- skeletal muscle
- anti inflammatory
- genome wide analysis
- nlrp inflammasome
- mesenchymal stem cells
- liver injury
- toll like receptor
- transcription factor