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High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery.

Jeremy TurchettoAna Filipa SequeiraLaurie RamondFanny PeyssonJoana L A BrásNatalie J SaezYoan DuhooMarilyne BlémontCatarina I P D GuerreiroLoic QuintonEdwin De PauwNicolas GillesHervé DarbonCarlos M G A FontesRenaud Vincentelli
Published in: Microbial cell factories (2017)
Overall this study reveals that high-throughput expression of animal venom peptides in E. coli can generate large libraries of recombinant disulphide-reticulated peptides of remarkable interest for drug discovery programs.
Keyphrases
  • drug discovery
  • high throughput
  • escherichia coli
  • poor prognosis
  • amino acid
  • single cell
  • public health
  • binding protein
  • staphylococcus aureus
  • cystic fibrosis
  • klebsiella pneumoniae
  • biofilm formation