Highly Efficient and Rapid Neural Differentiation of Mouse Embryonic Stem Cells Based on Retinoic Acid Encapsulated Porous Nanoparticle.
Se-Jin ParkSeongchan KimSung-Yon KimNoo Li JeonJoon Myong SongCheolhee WonDal-Hee MinPublished in: ACS applied materials & interfaces (2017)
An improved cell conversion strategy for neural differentiation of mouse embryonic stem (mES) cells is developed by incorporating functionalized mesoporous silica nanoparticle (MSN) as an efficient delivery carrier of retinoic acid (RA), which is a pleiotropic factor required for initiation of neural differentiation. Traditional RA-mediated neural differentiation methods required either preactivation of the cells to the differentiating state by embryoid body (EB) formation or repetitive treatment of the differentiation factor. Our modified cell conversion system involves only singular treatment of the RA/MSN complex, which simplified the whole process and accelerated neural induction to be finished within 6 days with high quality. With our new method, neural cells were successfully derived from mES cells with stable expression of neurite marker gene.
Keyphrases
- induced apoptosis
- cell cycle arrest
- highly efficient
- rheumatoid arthritis
- single cell
- endoplasmic reticulum stress
- cell death
- magnetic resonance imaging
- oxidative stress
- cell therapy
- signaling pathway
- poor prognosis
- gene expression
- systemic lupus erythematosus
- genome wide
- magnetic resonance
- pi k akt
- binding protein
- high frequency
- disease activity
- mesenchymal stem cells
- interstitial lung disease
- bone marrow
- computed tomography
- replacement therapy
- long non coding rna