Identification and Expression Analysis of MPK and MKK Gene Families in Pecan ( Carya illinoinensis ).
Juan ZhaoZong-Ming Max ChengMengyun ChenWenjuan MaJunping LiuPengpeng TanFangren PengPublished in: International journal of molecular sciences (2022)
Mitogen-activated protein kinases consist of three kinase modules composed of MPKs, MKKs, and MPKKKs. As members of the protein kinase (PK) superfamily, they are involved in various processes, such as developmental programs, cell division, hormonal progression, and signaling responses to biotic and abiotic stresses. In this study, a total of 18 MPKs and 10 MKKs were annotated on the pecan genome, all of which could be classified into four subgroups, respectively. The gene structures and conserved sequences of family members in the same branch were relatively similar. All MPK proteins had a conserved motif TxY, and D(L/I/V)K and VGTxxYMSPER existed in all MKK proteins. Duplication events contributed largely to the expansion of the pecan MPK and MKK gene families. Phylogenetic analysis of protein sequences from six plants indicated that species evolution occurred in pecan. Organ-specific expression profiles of MPK and MKK showed functional diversity. Ka / Ks values indicated that all genes with duplicated events underwent strong negative selection. Seven CiPawMPK and four CiPawMKK genes with high expression levels were screened by transcriptomic data from different organs, and these candidates were validated by qRT-PCR analysis of hormone-treated and stressed samples.
Keyphrases
- genome wide identification
- transcription factor
- genome wide
- protein kinase
- single cell
- poor prognosis
- copy number
- dna methylation
- genome wide analysis
- bioinformatics analysis
- public health
- cell therapy
- high resolution
- electronic health record
- gene expression
- adipose tissue
- stem cells
- type diabetes
- genetic diversity
- machine learning
- artificial intelligence
- amino acid
- protein protein
- insulin resistance
- network analysis