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Automation assisted anaerobic phenotyping for metabolic engineering.

Kaushik RajNaveen VenayakPatrick DiepSai Akhil GollaAlexander F YakuninRadhakrishnan Mahadevan
Published in: Microbial cell factories (2021)
Fixed-tip liquid handling systems can significantly reduce the amount of plastic waste generated in biological laboratories and our decontamination and calibration protocols could facilitate the widespread adoption of such systems. Further, the use of t-SNE in conjunction with our automation platform could serve as an effective scale-down model for bioreactor fermentations. Finally, by integrating an in-house data-analysis pipeline, we were able to accelerate the 'test' phase of the design-build-test-learn cycle of metabolic engineering.
Keyphrases
  • data analysis
  • wastewater treatment
  • high throughput
  • sewage sludge
  • microbial community
  • heavy metals
  • electronic health record
  • ionic liquid
  • saccharomyces cerevisiae
  • risk assessment
  • single cell