ELISpot Assay for Gene Therapy in Large Animal Studies.

Published in: Methods in molecular biology (Clifton, N.J.) (2022)

Formation of neutralizing antibodies and cellular immune response with repeat adeno-associated virus (AAV) gene therapy dosing are critical concerns in translational, large animal studies. The enzyme-linked immunospot/immunosorbent spot (ELISpot) assay introduced a way to track B- and/or T-cell response to therapy over time at a protein level. We describe the protocol for this assay looking at relative interferon (IFN)-γ secretion in pre- and post-AAV injections in a pig model.

Keyphrases

gene therapy
immune response
high throughput
dendritic cells
randomized controlled trial
case control
stem cells
ultrasound guided
single cell
binding protein
small molecule
platelet rich plasma
inflammatory response