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Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing.

Danielle N GallagherJames E Haber
Published in: ACS chemical biology (2017)
CRISPR/Cas9-mediated gene editing may involve nonhomologous end-joining to create various insertion/deletions (indels) or may employ homologous recombination to modify precisely the target DNA sequence. Our understanding of these processes has been guided by earlier studies using other site-specific endonucleases, both in model organisms such as budding yeast and in mammalian cells. We briefly review what has been gleaned from such studies using the HO and I-SceI endonucleases and how these findings guide current gene editing strategies.
Keyphrases
  • crispr cas
  • genome editing
  • dna repair
  • circulating tumor
  • dna damage
  • cell free
  • single molecule
  • case control
  • physical activity
  • mental health
  • nucleic acid
  • saccharomyces cerevisiae
  • gram negative
  • dna binding
  • high school