Mesenteric Lymph Duct Drainage Attenuates Lung Inflammatory Injury and Inhibits Endothelial Cell Apoptosis in Septic Rats.
Yongjun LiuChuanxi ChenQing SunHuadong SunNing LiuQier LiuJie MaPingping WangChunlin HuJianfeng WuBin OuyangJuan ChenMinying ChenXiang-Dong GuanPublished in: BioMed research international (2020)
The present study was to investigate the effect of mesenteric lymph duct drainage on lung inflammatory response, histological alteration, and endothelial cell apoptosis in septic rats. Animals were randomly assigned into four groups: control, sham surgery, sepsis, and sepsis plus mesenteric lymph drainage. We used the colon ascendens stent peritonitis (CASP) procedure to induce the septic model in rats, and mesenteric lymph drainage was performed with a polyethylene (PE) catheter inserted into mesenteric lymphatic. The animals were sacrificed at the end of CASP in 6 h. The mRNA expression levels of inflammatory mediators were measured by qPCR, and the histologic damage were evaluated by the pathological score method. It was found that mesenteric lymph drainage significantly reduced the expression of TNF-α, IL-1β, and IL-6 mRNA in the lung. Pulmonary interstitial edema and infiltration of inflammatory cells were alleviated by mesenteric lymph drainage. Moreover, increased mRNA levels of TNF-α, IL-1β, IL-6 mRNA, and apoptotic rate were observed in PMVECs treated with septic lymph. These results indicate that mesenteric lymph duct drainage significantly attenuated lung inflammatory injury by decreasing the expression of pivotal inflammatory mediators and inhibiting endothelial apoptosis to preserve the pulmonary barrier function in septic rats.
Keyphrases
- ultrasound guided
- acute kidney injury
- oxidative stress
- inflammatory response
- minimally invasive
- rheumatoid arthritis
- poor prognosis
- cell death
- cell cycle arrest
- endothelial cells
- cell proliferation
- intensive care unit
- pulmonary hypertension
- lymph node
- signaling pathway
- endoplasmic reticulum stress
- coronary artery disease
- atrial fibrillation
- long non coding rna
- percutaneous coronary intervention
- lps induced