Photo-Oxidizing Ruthenium(II) Complexes with Enhanced Visible-Light Absorption and G-quadruplex DNA Binding Abilities.
Martin GillardGuillaume PirauxMartin DaenenMichaël AbrahamLudovic Troian-GautierLaure BarHugues BonnetFrédérique LoiseauHélène JametJérôme DejeuEric DefrancqBenjamin EliasPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2022)
Photosensitizers that gather high photo-oxidizing power and strong visible-light absorption are of great interest in the development of new photo-chemotherapeutics. Indeed, such compounds constitute attractive candidates for the design of type I photosensitizers that are not dependent on the presence of oxygen. In this paper, we report on the synthesis and studies of new ruthenium(II) complexes that display strong visible-light absorption and can oxidize guanine residues under visible-light irradiation, as evidenced by nanosecond transient absorption spectroscopy. The reported compounds also tightly bind to G-quadruplex DNA structures from the human telomeric sequence (TTAGGG repeat). The kinetic and thermodynamic parameters of the interaction of these Ru(II) complexes with G-quadruplex and duplex DNA were studied thanks to luminescence titrations and bio-layer interferometry measurements, which revealed higher affinities towards the non-canonical G-quadruplex architecture. Docking experiments and non-covalent ionic analysis allowed us to gain information on the mode and the strength of the interaction of the compounds towards G-quadruplex and duplex DNA. The different studies emphasize the substantial influence of the position and the number of non-chelating nitrogen atoms on the interaction with both types of DNA secondary structures.
Keyphrases
- visible light
- circulating tumor
- single molecule
- cell free
- dna binding
- photodynamic therapy
- high resolution
- endothelial cells
- healthcare
- solid state
- molecular dynamics
- molecular dynamics simulations
- circulating tumor cells
- dna damage
- single cell
- protein protein
- oxidative stress
- subarachnoid hemorrhage
- pluripotent stem cells