Integrative Analysis of lncRNA-mRNA Coexpression in Human Lung Epithelial Cells Exposed to Dimethyl Selenide-Derived Secondary Organic Aerosols.
C M Sabbir AhmedBiplab Chandra PaulYumeng CuiAlexander L FrieAbigail BurrRohan KamathJin Y ChenTara M NordgrenRoya BahreiniYing-Hsuan LinPublished in: Chemical research in toxicology (2021)
Dimethyl selenide (DMSe) is one of the major volatile organoselenium compounds released into the atmosphere through plant metabolism and microbial methylation. DMSe has been recently revealed as a precursor of secondary organic aerosol (SOA), and its resultant SOA possesses strong oxidizing capability toward thiol groups that can perturb several major biological pathways in human airway epithelial cells and is linked to genotoxicity, DNA damage, and p53-mediated stress responses. Mounting evidence has suggested that long noncoding RNAs (lncRNAs) are involved in stress responses to internal and environmental stimuli. However, the underlying molecular interactions remain to be elucidated. In this study, we performed integrative analyses of lncRNA-mRNA coexpression in the transformed human bronchial epithelial BEAS-2B cell line exposed to DMSe-derived SOA. We identified a total of 971 differentially expressed lncRNAs in BEAS-2B cells exposed to SOA derived from O3 and OH oxidation of DMSe. Gene ontology (GO) network analysis of cis-targeted genes showed significant enrichment of DNA damage, apoptosis, and p53-mediated stress response pathways. trans-Acting lncRNAs, including PINCR, PICART1, DLGAP1-AS2, and LINC01629, known to be associated with human carcinogenesis, also showed altered expression in cell treated with DMSe-SOA. Overall, this study highlights the regulatory role of lncRNAs in altered gene expression induced by DMSe-SOA exposure.
Keyphrases
- network analysis
- dna damage
- endothelial cells
- gene expression
- genome wide identification
- oxidative stress
- long non coding rna
- induced pluripotent stem cells
- genome wide
- genome wide analysis
- dna methylation
- dna repair
- poor prognosis
- long noncoding rna
- binding protein
- transcription factor
- cell death
- mass spectrometry
- stem cells
- cell proliferation
- endoplasmic reticulum stress
- single molecule
- life cycle