Determination of Alternaria Mycotoxins in Fresh Sweet Cherries and Cherry-Based Products: Method Validation and Occurrence.
Xiaoting QiaoJie YinYunjia YangJing ZhangBing ShaoHui LiHongbing ChenPublished in: Journal of agricultural and food chemistry (2018)
Sweet cherry is susceptible to disease caused by the Alternaria species and produces various Alternaria mycotoxins. Analytical methodologies based on solid-phase extraction (SPE) and LC-MS/MS to simultaneously determine five main Alternaria mycotoxins (tenuazonic acid, 1; alternariol, 2; alternariol methyl ether, 3; altenuene, 4; and tentoxin, 5) in fresh sweet cherries and cherry products were developed and validated. The limits of quantitation (LOQ) of the analytes ranged from 0.002-0.066 μg/kg. The method was successfully applied to 83 fresh cherry and cherry-related product samples. 1 and 5 were the predominant toxins with detection frequencies >50%, followed by 3 (42%), 2 (35%), and 4 (31%). Daily intakes of Alternaria mycotoxins via fresh sweet cherries were assessed preliminarily using the measured concentrations, and consumption data were obtained from a web-based dietary questionnaire ( n = 476). The maximum exposure of 1 and 3 were 4.6 and 16.7 times the threshold of the toxicological concern (TTC) value, respectively.
Keyphrases
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- high performance liquid chromatography
- molecularly imprinted
- liquid chromatography
- tandem mass spectrometry
- simultaneous determination
- gas chromatography mass spectrometry
- ms ms
- ultra high performance liquid chromatography
- mass spectrometry
- gas chromatography
- cross sectional
- ionic liquid
- machine learning
- quantum dots
- genetic diversity
- patient reported
- real time pcr