Optimized reporters for multiplexed detection of transcription factor activity.

Max TrauernichtTeodora FilipovskaChaitanya Rastogi Bas van Steensel

Published in: bioRxiv : the preprint server for biology (2024)

Systematic design and optimization of transcriptional reporters for 86 TFsCharacterization of TF-specific reporter design optimization rulesEvaluation of reporter TF-specificity across a wide array of TF perturbationsIdentification of a collection of 60 "prime" TF reporters with optimized performance.

Keyphrases

transcription factor
crispr cas
gene expression
high resolution
dna binding
loop mediated isothermal amplification
mass spectrometry
oxidative stress
real time pcr
heat shock
heat shock protein