Canavalia gladiata Pod Extract Mitigates Ovalbumin-Induced Asthma Onset in Male BALB/c Mice via Suppression of MAPK.
Wen Yan HuangSang Hoon LeeSeong Ju OhHyeock YoonJeong-Hoon PanInhye JeongMi Jeong KimBok Kyung HanJae Kyeom KimEui-Cheol ShinYoung Jun KimPublished in: Molecules (Basel, Switzerland) (2022)
Asthma is one of the most common inflammatory diseases of the lung worldwide. There has been considerable progress in recent studies to treat and prevent allergic asthma, however, various side effects are still observed in clinical practice. Six-week-old male BALB/c mice were orally administered with either sword bean pod extracts (SBP; 100 or 300 mg/kg) or dexamethasone (DEX; 5 mg/kg) once daily over 3 weeks, followed by ovalbumin sensitization (OVA/Alum.; intraperitoneal administration, 50 μg/2 mg/per mouse). Scoring of lung inflammation was performed to observe pathological changes in response to SBP treatment compared to OVA/Alum.-induced lung injury. Additionally, inflammatory cytokines were quantified in serum, bronchoalveolar lavage fluid (BALF), and lung tissue using ELISA and Western blot analyses. SBP treatment significantly reduced the infiltration of inflammatory cells, and release of histamine, immunoglobulin E, and leukotriene in serum and BALF. Moreover, the therapeutic effect of SBP was also assessed to analyze the inflammatory changes in the lung tissues. SBP markedly suppressed the activation of the MAPK signaling pathway and the expression of key inflammatory proteins (e.g., TNF-α) and Th2 type cytokines (IL-5 and IL-13). SBP was effective in ameliorating the allergic inflammation against OVA/Alum.-induced asthma by suppressing pulmonary inflammation.
Keyphrases
- oxidative stress
- diabetic rats
- induced apoptosis
- allergic rhinitis
- signaling pathway
- chronic obstructive pulmonary disease
- lung function
- high glucose
- clinical practice
- pi k akt
- gene expression
- pulmonary hypertension
- drug induced
- rheumatoid arthritis
- poor prognosis
- low dose
- type diabetes
- air pollution
- high dose
- cell cycle arrest
- long non coding rna
- south africa
- insulin resistance
- metabolic syndrome
- cell proliferation
- endoplasmic reticulum stress
- wild type
- case control