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SenNet recommendations for detecting senescent cells in different tissues.

Vidyani SuryadevaraAdam D HudginsAdarsh RajeshAlberto PappalardoAlla KarpovaAmit Kumar DeyAnn HertzelAnthony AgudeloAzucena RochaBikem SoygurBirgit SchillingChase M CarverCristina Aguayo-MazzucatoDarren J BakerDavid A BernlohrDiana JurkDilyana B MangarovaEllen M QuardokusElizabeth Ann L EnningaElizabeth L SchmidtFeng ChenFrancesca E DuncanFrancesco CambuliGagandeep KaurGeorge A KuchelGung LeeHeike E Daldrup-LinkHelene MartiniHemali PhatnaniIman M Al-NaggarIrfan RahmanJia NieJoão F PassosJonathan C SilversteinJudith CampisiJulia WangKanako IwasakiKarina BarbosaKay MetisKerem NernekliLaura J NiedernhoferLi DingLichao WangLisa C AdamsLiu RuiyangMadison L DoolittleMarcos G TenecheMarissa J SchaferMing XuMohammadjavad HajipourMozhgan BoroumandNathan BasistyNicholas SloanNikolai SlavovOlena KuksenkoPaul RobsonPaul T GomezPeriklis VasilikosPeter D AdamsPriscila CarapetoQuan ZhuRamalakshmi RamasamyRolando Perez-LorenzoRong FanRunze DongRuth Rebecca MontgomerySadiya ShaikhSanja VickovicShanshan YinShoukai KangSonja SuvakovSundeep KhoslaVesna D GarovicVilas MenonYanxin XuYizhe SongYousin SuhZhixun DouNicola Neretti
Published in: Nature reviews. Molecular cell biology (2024)
Once considered a tissue culture-specific phenomenon, cellular senescence has now been linked to various biological processes with both beneficial and detrimental roles in humans, rodents and other species. Much of our understanding of senescent cell biology still originates from tissue culture studies, where each cell in the culture is driven to an irreversible cell cycle arrest. By contrast, in tissues, these cells are relatively rare and difficult to characterize, and it is now established that fully differentiated, postmitotic cells can also acquire a senescence phenotype. The SenNet Biomarkers Working Group was formed to provide recommendations for the use of cellular senescence markers to identify and characterize senescent cells in tissues. Here, we provide recommendations for detecting senescent cells in different tissues based on a comprehensive analysis of existing literature reporting senescence markers in 14 tissues in mice and humans. We discuss some of the recent advances in detecting and characterizing cellular senescence, including molecular senescence signatures and morphological features, and the use of circulating markers. We aim for this work to be a valuable resource for both seasoned investigators in senescence-related studies and newcomers to the field.
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