Evaluation of different IRES-mediated tricistronic plasmid designs for expression of an anti-PCSK9 biosimilar monoclonal antibody in CHO cells.

Thayana A CruzMarcos B PinhoLeda R Castilho

Published in: Biotechnology letters (2020)

The expression platform proposed showed to be efficient to produce a high-quality anti-PCSK9 mAb in stable CHO cell pools and provides benchmarks for fast production of different mAbs for characterization, formulation studies and pre-clinical investigation.

Keyphrases

monoclonal antibody
poor prognosis
induced apoptosis
escherichia coli
single cell
binding protein
drug delivery
long non coding rna
high throughput
stem cells
endoplasmic reticulum stress
cell proliferation
case control