SERS spectroscopy with machine learning to analyze human plasma derived sEVs for coronary artery disease diagnosis and prognosis.
Xi HuangBo LiuShenghan GuoWeihong GuoKe LiaoGuoku HuWen ShiMitchell KussMichael J DuryeeDaniel R AndersonYong Feng LuBin DuanPublished in: Bioengineering & translational medicine (2022)
Coronary artery disease (CAD) is one of the major cardiovascular diseases and represents the leading causes of global mortality. Developing new diagnostic and therapeutic approaches for CAD treatment are critically needed, especially for an early accurate CAD detection and further timely intervention. In this study, we successfully isolated human plasma small extracellular vesicles (sEVs) from four stages of CAD patients, that is, healthy control, stable plaque, non-ST-elevation myocardial infarction, and ST-elevation myocardial infarction. Surface-enhanced Raman scattering (SERS) measurement in conjunction with five machine learning approaches, including Quadratic Discriminant Analysis, Support Vector Machine (SVM), K-Nearest Neighbor, Artificial Neural network, were then applied for the classification and prediction of the sEV samples. Among these five approaches, the overall accuracy of SVM shows the best predication results on both early CAD detection (86.4%) and overall prediction (92.3%). SVM also possesses the highest sensitivity (97.69%) and specificity (95.7%). Thus, our study demonstrates a promising strategy for noninvasive, safe, and high accurate diagnosis for CAD early detection.
Keyphrases
- coronary artery disease
- percutaneous coronary intervention
- st elevation myocardial infarction
- machine learning
- cardiovascular events
- coronary artery bypass grafting
- neural network
- end stage renal disease
- label free
- high resolution
- cardiovascular disease
- gold nanoparticles
- deep learning
- randomized controlled trial
- chronic kidney disease
- aortic stenosis
- newly diagnosed
- sensitive detection
- ejection fraction
- metabolic syndrome
- acute coronary syndrome
- prognostic factors
- loop mediated isothermal amplification
- patient reported outcomes
- atrial fibrillation
- type diabetes
- risk factors
- mass spectrometry
- smoking cessation