Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor.
Yang TianChien-Yuan LinJoon-Hyun ParkChuan-Yin WuRamu KakumanuVenkataramana R PidatalaKhanh M VuuAlberto RodriguezPatrick M ShihEdward E K BaidooStephen TempleBlake A SimmonsJohn M GladdenHenrik V SchellerAymerick EudesPublished in: Biotechnology for biofuels (2021)
Expression of OsAT10 in sorghum leads to an increase of xylan-bound pCA without reducing the overall content of cell wall FA esters. Nevertheless, the amount of total cell wall pCA remains unchanged indicating that most pCA is ester-linked to lignin. Unlike other engineered plants overexpressing OsAT10 or a phylogenetically related acyltransferase with similar putative function, the improvements of biomass saccharification efficiency in sorghum OsAT10 lines are likely the result of lignin reductions rather than reductions of cell wall-bound FA. These results also suggest a relationship between xylan-bound pCA and lignification in cell walls.