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A brief overview on the application and sources of α-amylase and expression hosts properties in order to production of recombinant α-amylase.

Ahmad MovahedpourMarzieh AsadiSeyyed Hossein KhatamiMehrnoosh Azimi SanaviMaryam AdelipourZahra ShabaninejadNahid AhmadiCambyz IrajieMousavi Pegah
Published in: Biotechnology and applied biochemistry (2021)
By reducing the activation energy, enzymes accelerate the chemical reaction; therefore, they are good alternative for industrial catalysts. Amylase is a suitable enzyme as a catalyst for the chemical decomposition of starch. This enzyme is of great importance, and its production is highly profitable. α-Amylase is among the most important amylases produced naturally by animals, plants, and microorganisms. Still, the α-amylases produced by bacteria have a special place in industry and commerce. Moreover, a large volume of this enzyme can be produced by selecting an appropriate and optimized host to clone and express the α-amylase gene. The present study briefly reviews the structure, application, sources, and hosts used to produce recombinant α-amylase.
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