GALAXY ver3: updated web application for glycosylation profiling based on 3D HPLC map.
Hirokazu YagiEijiro AmagasaMasaaki ShiotaIssaku YamadaKiyoko F Aoki-KinoshitaKoichi KatoPublished in: Glycobiology (2022)
High-performance liquid chromatography (HPLC) elution data provide a useful tool for quantitative glycosylation profiling, discriminating isomeric oligosaccharides. The web application Glycoanalysis by the Three Axes of MS and Chromatography (GALAXY), which is based on the three-dimensional HPLC map of N-linked oligosaccharides with pyridyl-2-amination developed by Dr. Noriko Takahashi, has been extensively used for N-glycosylation profiling at molecular, cellular, and tissue levels. Herein, we describe the updated GALAXY as version 3, which includes new HPLC data including those of glucuronylated and sulfated glycans, an improved graphical user interface using modern technologies, and linked to glycan information in GlyTouCan and the GlyCosmos Portal. This liaison will facilitate glycomic analyses of human and other organisms in conjunction with multiomics data.
Keyphrases
- high performance liquid chromatography
- simultaneous determination
- ms ms
- mass spectrometry
- tandem mass spectrometry
- solid phase extraction
- liquid chromatography
- electronic health record
- big data
- single cell
- high resolution
- endothelial cells
- multidrug resistant
- multiple sclerosis
- single molecule
- data analysis
- health information
- gram negative
- psychometric properties