Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection.

Rujian ZhaoChunxu YuBaiyang LuBingling Li

Published in: RSC advances (2022)

We report a universal and signal-on HCR based detection platform via innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practical sensitivity as low as ∼aM of ASFV gene in serum.

Keyphrases

crispr cas
genome editing
nucleic acid
room temperature
loop mediated isothermal amplification
genome wide
high throughput
label free
real time pcr
dna methylation
transcription factor
genome wide identification
gene expression
ionic liquid