Optogenetic manipulation has proven a powerful tool for investigating the mechanisms underlying the function of neuronal networks, but implementing the technique on mammals during early development remains challenging. Here, we present a comprehensive workflow to specifically manipulate mitral/tufted cells (M/TCs), the output neurons in the olfactory circuit, mediated by adeno-associated virus (AAV) transduction and light stimulation in neonatal mice and monitor neuronal and network activity with in vivo electrophysiology. This method represents an efficient approach to elucidate functional brain development. For complete details on the use and execution of this protocol, please refer to Chen et al. 1 , 2 , 3 .
Keyphrases
- gene therapy
- randomized controlled trial
- cerebral ischemia
- induced apoptosis
- high fat diet induced
- spinal cord
- mitral valve
- left ventricular
- cell cycle arrest
- white matter
- type diabetes
- left atrial
- multiple sclerosis
- quality improvement
- wild type
- heart failure
- electronic health record
- signaling pathway
- cell death
- metabolic syndrome
- spinal cord injury
- brain injury
- transcatheter aortic valve replacement
- pi k akt