Visceral Adipose Tissue Bioenergetics Varies According to Individuals' Obesity Class.
Marcelo V TopeteSara AndradeRaquel L BernardinoMarta GuimarãesAna M PereiraSofia B OliveiraMadalena M CostaMário NoraMariana P MonteiroSofia S PereiraPublished in: International journal of molecular sciences (2023)
Obesity is associated with complex adipose tissue energy metabolism remodeling. Whether AT metabolic reprogramming differs according to body mass index (BMI) and across different obesity classes is unknown. This study’s purpose was to evaluate and compare bioenergetics and energy substrate preference of visceral adipose tissue (VAT) pertaining to individuals with obesity class 2 and class 3. VAT obtained from patients with obesity (n = 15) class 2 (n = 7; BMI 37.53 ± 0.58 kg/m2) or class 3 (n = 8; BMI 47.79 ± 1.52 kg/m2) was used to assess oxygen consumption rate (OCR) bioenergetics and mitochondrial substrate preferences. VAT of patients with obesity class 3 presented significantly higher non-mitochondrial oxygen consumption (p < 0.05). In VAT of patients with obesity class 2, inhibition of pyruvate and glutamine metabolism significantly decreased maximal respiration and spare respiratory capacity (p < 0.05), while pyruvate and fatty acid metabolism inhibition, which renders glutamine the only available substrate, increased the proton leak with a protective role against oxidative stress (p < 0.05). In conclusion, VAT bioenergetics of patients with obesity class 2 depicts a greater dependence on glucose/pyruvate and glutamine metabolism, suggesting that patients within this BMI range are more likely to be responsive to interventions based on energetic substrate modulation for obesity treatment.
Keyphrases
- insulin resistance
- weight gain
- adipose tissue
- metabolic syndrome
- body mass index
- weight loss
- high fat diet induced
- type diabetes
- oxidative stress
- high fat diet
- skeletal muscle
- physical activity
- chronic kidney disease
- fatty acid
- end stage renal disease
- signaling pathway
- drug delivery
- dna damage
- cancer therapy
- induced apoptosis
- combination therapy
- respiratory tract