Mangiferin improves early porcine embryonic development by reducing oxidative stress.
He-Wei JiChao-Rui WangXiu-Wen YuanJing WangLin WangQi-Long CaoYing-Hua LiYong-Nan XuNam-Hyung KimPublished in: Reproduction in domestic animals = Zuchthygiene (2024)
Mangiferin (MGN) is primarily found in the fruits, leaves, and bark of plants of the Anacardiaceae family, including mangoes. MGN exhibits various pharmacological effects, such as protection of the liver and gallbladder, anti-lipid peroxidation, and cancer prevention. This study aimed to investigate the effects of MGN supplementation during in vitro culture (IVC) on the antioxidant capacity of early porcine embryos and the underlying mechanisms involved. Porcine parthenotes in the IVC medium were exposed to different concentrations of MGN (0, 0.01, 0.1, and 1 μM). The addition of 0.1 μM MGN significantly increased the blastocyst formation rate of porcine embryos while reducing the apoptotic index and autophagy. Furthermore, the expression of antioxidation-related (SOD2, GPX1, NRF2, UCHL1), cell pluripotency (SOX2, NANOG), and mitochondria-related (TFAM, PGC1α) genes was upregulated. In contrast, the expression of apoptosis-related (CAS3, BAX) and autophagy-related (LC3B, ATG5) genes decreased after MGN supplementation. These findings suggest that MGN improves early porcine embryonic development by reducing oxidative stress-related genes.
Keyphrases
- oxidative stress
- cell death
- endoplasmic reticulum stress
- stem cells
- ischemia reperfusion injury
- inferior vena cava
- genome wide
- diabetic rats
- skeletal muscle
- computed tomography
- crispr cas
- transcription factor
- cell therapy
- gene expression
- single cell
- binding protein
- cell cycle arrest
- mesenchymal stem cells
- fatty acid
- simultaneous determination
- amyotrophic lateral sclerosis
- young adults
- drug induced
- heat shock protein