Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis.

Dingyu LiuCan HuangJiaxin GuoPeiji ZhangTao ChenZhi-Wen WangXueming Zhao

Published in: Biotechnology for biofuels (2019)

We present not only the iterative CRISPR/Cas9n system for B. subtilis but also the highest efficiency for simultaneous modulation of multiple genes on the chromosome in B. subtilis reported to date. We anticipate this CRISPR/Cas9n mediated system to greatly enhance the optimization of diverse biological systems via metabolic engineering and synthetic biology.

Keyphrases

crispr cas
genome editing
bacillus subtilis
genome wide
magnetic resonance imaging
high throughput
image quality
magnetic resonance
gene expression
bioinformatics analysis
single cell
real time pcr
contrast enhanced