GuideMaker: Software to design CRISPR-Cas guide RNA pools in non-model genomes.
Ravin PoudelLidimarie Trujillo RodriguezChristopher R ReischAdam R RiversPublished in: GigaScience (2022)
GuideMaker enables the rapid design of genome-wide gRNA for any CRISPR-Cas enzyme in non-model organisms. While GuideMaker is designed with prokaryotic genomes in mind, it can efficiently process eukaryotic genomes as well. GuideMaker is available as command-line software, a stand-alone web application, and a tool in the CyCverse Discovery Environment. All versions are available under a Creative Commons CC0 1.0 Universal Public Domain Dedication.