Mitochondria-Targeted Ratiometric Chemdosimeter to Detect Hypochlorite Acid for Monitoring the Drug-Damaged Liver and Kidney.
Lina ShangguanJing WangXiaoli QianYong-Quan WuYi LiuPublished in: Analytical chemistry (2022)
Liver and kidney injury caused by drug toxicity is a serious threat to human health. Acetaminophenol (APAP), as a common antipyretic and analgesic drug, inevitably causes injury. When it is overused, hypochlorous acid (HClO) is excessively generated due to metabolic abnormalities, resulting in the accumulation of HClO in the mitochondria of liver and kidney tissues and causing damage. In this study, we designed a series of HClO responsive ratiometric chemdosimeter NRH-X (NRH-O, NRH-S, and NRH-C) to evaluate liver and kidney injury, and found that NRH-O has a specific sensitive response to HClO. NRH-O can not only monitor the variations of endogenous HClO content of living cells by fluorescence ratio changes in the mitochondria but also detect the upregulation of HClO induced by APAP. In addition, NRH-O can also be used for anatomic diagnosis of liver and kidney injury by fluorescence ratio imaging of HClO in the tissues of inflammatory mice.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- human health
- oxidative stress
- risk assessment
- gene expression
- cancer therapy
- climate change
- cell death
- high resolution
- energy transfer
- sensitive detection
- quantum dots
- spinal cord injury
- metabolic syndrome
- type diabetes
- long non coding rna
- poor prognosis
- emergency department
- hydrogen peroxide
- drug induced
- skeletal muscle
- nitric oxide
- insulin resistance
- drug delivery
- adverse drug
- anti inflammatory
- photodynamic therapy