Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS Exposure.
Henry Velázquez-SotoFernanda Real-San MiguelSonia Mayra Pérez-TapiaMaría Del Carmen Jiménez-MartínezPublished in: Biomolecules (2022)
A key aspect of the inflammatory phenomenon is the involvement of costimulatory molecules expressed by antigen-presenting cells (APCs) and their ability to secrete cytokines to set instructions for an adaptive immune response and to generate tolerance or inflammation. In a novel integrative approach, we aimed to evaluate the kinetic expression of the membrane and soluble B7 costimulatory molecules CD86, ICOS-L, PDL1, PDL2, the transcription factor Interferon Regulatory Factor 4 (IRF4), and the cytokines produced by monocyte-derived dendritic cells (Mo-DCs) after challenging them with different concentrations of stimulation with E. coli lipopolysaccharide (LPS) for different lengths of time. Our results showed that the stimuli concentration and time of exposure to an antigen are key factors in modulating the dynamic expression pattern of membrane and soluble B7 molecules and cytokines.
Keyphrases
- dendritic cells
- immune response
- poor prognosis
- transcription factor
- regulatory t cells
- inflammatory response
- oxidative stress
- endothelial cells
- binding protein
- induced apoptosis
- long non coding rna
- anti inflammatory
- signaling pathway
- cell death
- endoplasmic reticulum stress
- induced pluripotent stem cells
- network analysis