Login / Signup

CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells.

Mateusz KotowskiSumana Sharma
Published in: Methods and protocols (2020)
While clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing techniques have been widely adapted for use in immortalised immune cells, efficient manipulation of primary T cells has proved to be more challenging. Nonetheless, the rapid expansion of the CRISPR toolbox accompanied by the development of techniques for delivery of CRISPR components into primary T cells now affords the possibility to genetically manipulate primary T cells both with precision and at scale. Here, we review the key features of the techniques for primary T cell editing and discuss how the new generation of CRISPR-based tools may advance genetic engineering of these immune cells. This improved ability to genetically manipulate primary T cells will further enhance our fundamental understanding of cellular signalling and transcriptional networks in T cells and more importantly has the potential to revolutionise T cell-based therapies.
Keyphrases
  • crispr cas
  • genome editing
  • genome wide
  • risk assessment
  • oxidative stress
  • climate change
  • sensitive detection
  • heat shock