Therapeutic Potential of Two Visible Light Responsive Luminescent photoCORMs: Enhanced Cellular Internalization Driven by Lipophilicity.
Miguel N PintoIndranil ChakrabortyJorge JimenezKatelyn MurphyJohn WengerPradip K MascharakPublished in: Inorganic chemistry (2019)
Herein we report the synthesis, characterization, and cellular internalization properties of two visible-light active luminescent Mn-based photoCORMs. The enhanced membrane permeability of the photoactive Mn carbonyl complex (photoCORM) derived from a designed lipophilic ligand namely, [Mn(CO)3(Imdansyl)(L1)](CF3SO3) (1) (where L1 = a diazabutadiene-based ligand containing two highly lipophilic adamantyl motifs, Imdansyl = dansylimidazole) promoted rapid internalization within human colorectal adenocarcinoma (HT-29) cells compared to [Mn(CO)3(Imdansyl)(L2)](CF3SO3) (2) (where L2 = a diazabutadiene ligand bearing two hydrophilic 1,3,5-triazaadamantyl group). Colocalization experiments using membrane stain indicate different extents of localization of the two CO complexes within the cellular matrix. Visible-light triggered CO release from the lipophilic photoCORM induced caspase-3/7 activation on HT-29 cells, which was detected using confocal microscopy. The rapid accumulation of the lipophilic photoCORM 1 in the cellular membrane resulted in more efficient CO-induced cell death compared to the hydrophilic analogue 2.
Keyphrases
- visible light
- cell death
- induced apoptosis
- cell cycle arrest
- metal organic framework
- endothelial cells
- high glucose
- cystic fibrosis
- room temperature
- diabetic rats
- endoplasmic reticulum stress
- squamous cell carcinoma
- oxidative stress
- quantum dots
- transition metal
- liquid chromatography
- drug induced
- loop mediated isothermal amplification
- pi k akt
- cell proliferation
- cancer therapy
- energy transfer
- ionic liquid
- stress induced
- rectal cancer
- pluripotent stem cells