Canniprene B, a new prenylated dihydrostilbene with cytotoxic activities from the leaves of Cannabis sativa .

A new, canniprene B ( 4 ), along with five known ( 1 - 3 and 5 - 6 ) dihydrostilbenes were isolated from the leaves of Cannabis sativa collected at CSIR - IIIM, Jammu, India. Structures of all isolated compounds were elucidated by spectroscopic data analysis, including 1D and 2D NMR, and HR-ESI-MS. Canniprene B is a new prenylated dihydrostilbenes, a positional isomer of the known compound canniprene ( 5 ). The cytotoxic activities of these compounds ( 1 - 6 ) were evaluated using the SRB assay against a panel of five human cancer cell lines. Notably, canniprene B ( 4 ) exhibited varying levels of cytotoxicity with IC 50 values ranging from 2.5 to 33.52 μM, demonstrating the most potent activity against pancreatic cancer cells.