Engineering threshold-based selection systems.

Katherine H PedoneVanessa González-PérezLuciana E LeopoldNeal R RasmussenChanning J DerAdrienne D CoxShawn Ahmed David J Reiner

Published in: G3 (Bethesda, Md.) (2021)

Using model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt Caenorhabditis elegans embryonic morphogenesis, titrated to 100% lethality. The strategy of engineering thresholds for positive selection using experimental animals was validated with pharmacological and genetic suppression and is generalizable to diverse molecular processes and experimental systems.

Keyphrases

genome wide
poor prognosis
copy number
gene expression
gram negative