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Gene regulatory network controlling carpel number variation in cucumber.

Gen CheRan GuJianyu ZhaoXiaofeng LiuXiaofei SongHailing ZiZhihua ChengJunjun ShenZhongyi WangRenyi LiuLiying YanYiqun WengXiaolan Zhang
Published in: Development (Cambridge, England) (2020)
The WUSCHEL-CLAVATA3 pathway genes play an essential role in shoot apical meristem maintenance and floral organ development, and under intense selection during crop domestication. The carpel number is an important fruit trait that affects fruit shape, size and internal quality in cucumber, but the molecular mechanism remains elusive. Here, we found that CsCLV3 expression was negatively correlated with carpel number in cucumber cultivars. CsCLV3-RNAi led to increased number of petals and carpels, whereas overexpression of CsWUS resulted in more sepals, petals and carpels, suggesting that CsCLV3 and CsWUS function as a negative and a positive regulator for carpel number variation, respectively. Biochemical analyses indicated that CsWUS directly bound to the promoter of CsCLV3 and activated its expression. Overexpression of CsFUL1A , a FRUITFULL-like MADS-box gene, resulted in more petals and carpels. CsFUL1A can directly bind to the CsWUS promoter to stimulate its expression. Furthermore, we found that auxin participated in carpel number variation in cucumber through interaction of CsARF14 with CsWUS. Therefore, we have identified a gene regulatory pathway involving CsCLV3, CsWUS, CsFUL1A and CsARF14 in determining carpel number variation in an important vegetable crop - cucumber.
Keyphrases
  • transcription factor
  • poor prognosis
  • dna methylation
  • genome wide
  • binding protein
  • gene expression
  • cell proliferation
  • climate change
  • genome wide identification
  • long non coding rna
  • copy number
  • arabidopsis thaliana