Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences.
Rebekka M KoeckFlorence BusatoJorg TostDimitri ConstenJannie van Echten-ArendsSebastiaan MastenbroekYvonne WurthSylvie RemySabine LangieTim S NawrotMichelle PlusquinRossella AlfanoEsmée M BijnensMarij GielenRon van GoldeJohn C M DumoulinHan BrunnerAafke P A van MontfoortMasoud Zamani EstekiPublished in: NPJ genomic medicine (2022)
A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration.
Keyphrases
- pluripotent stem cells
- gestational age
- dna methylation
- birth weight
- pregnancy outcomes
- genome wide
- preterm birth
- umbilical cord
- pregnant women
- low birth weight
- mesenchymal stem cells
- gene expression
- weight gain
- young adults
- preterm infants
- endothelial cells
- emergency department
- physical activity
- adipose tissue
- bone marrow
- weight loss
- high resolution
- metabolic syndrome
- liquid chromatography
- induced pluripotent stem cells