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Xeno-free generation of human induced pluripotent stem cells from donor-matched fibroblasts isolated from dermal and oral tissues.

Hassan R W AliSalwa SulimanTarig Al-Hadi OsmanManuel CarrascoOve BrulandDaniela-Elena CosteaHelge RæderKamal Mustafa
Published in: Stem cell research & therapy (2023)
The xeno-free protocol had a lower reprogramming efficiency compared to the standard xenogenic protocol. The oral fibroblasts generally proved to be more difficult to reprogram than dermal fibroblasts. Xeno-free dermal, buccal and gingival fibroblasts can successfully generate iPS with a comparable genotype/phenotype to their xenogenic counterparts.
Keyphrases
  • induced pluripotent stem cells
  • extracellular matrix
  • randomized controlled trial
  • endothelial cells
  • gene expression
  • wound healing