Xeno-free generation of human induced pluripotent stem cells from donor-matched fibroblasts isolated from dermal and oral tissues.
Hassan R W AliSalwa SulimanTarig Al-Hadi OsmanManuel CarrascoOve BrulandDaniela-Elena CosteaHelge RæderKamal MustafaPublished in: Stem cell research & therapy (2023)
The xeno-free protocol had a lower reprogramming efficiency compared to the standard xenogenic protocol. The oral fibroblasts generally proved to be more difficult to reprogram than dermal fibroblasts. Xeno-free dermal, buccal and gingival fibroblasts can successfully generate iPS with a comparable genotype/phenotype to their xenogenic counterparts.