Cryopreservation of human cancers conserves tumour heterogeneity for single-cell multi-omics analysis.
Sunny Z WuDaniel L RodenGhamdan Al-EryaniNenad BartonicekKate HarveyAurélie S CazetChia-Ling ChanSimon JunankarMun N HuiEwan A MillarJulia BeretovLisa HorvathAnthony M JoshuaPhillip StrickerJames S WilmottCamelia QuekGeorgina V LongRichard A ScolyerBertrand Z YeungDavendra SegaraCindy MakSanjay WarrierJoseph E PowellSandra O'TooleElgene LimAlexander SwarbrickPublished in: Genome medicine (2021)
We show that the viable cryopreservation of human cancers provides high-quality single-cells for multi-omics analysis. Our study guides new experimental designs for tissue biobanking for future clinical single-cell RNA sequencing studies.