Analysis of copy number variations induced by ultrashort electron beam radiation in human leukocytes in vitro.
Tigran HarutyunyanGalina HovhannisyanAnzhela SargsyanBagrat GrigoryanAhmed H Al-RikabiAnja WeiseThomas LiehrRouben AroutiounianPublished in: Molecular cytogenetics (2019)
In this study we demonstrated that irradiation with laser-driven electron bunches can induce molecular-cytogenetically visible CNVs in human blood leukocytes in vitro. These CNVs occur most likely due to duplications or amplification and tend to inversely correlate with chromosome size and gene density. CNVs can last in cell population as stable chromosomal changes for several days after radiation exposure; therefore this endpoint can be used for characterization of genetic effects of accelerated electrons. These findings should be complemented with other studies and implementation of more sophisticated approaches for CNVs analysis.
Keyphrases
- copy number
- mitochondrial dna
- genome wide
- endothelial cells
- dna methylation
- induced pluripotent stem cells
- healthcare
- pluripotent stem cells
- peripheral blood
- primary care
- single cell
- gene expression
- mass spectrometry
- stem cells
- high resolution
- mesenchymal stem cells
- bone marrow
- transcription factor
- quality improvement
- high speed