Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy.
Zhengyang ZhangSamuel J KennyMargaret HauserWan LiKe XuPublished in: Nature methods (2015)
By developing a wide-field scheme for spectral measurement and implementing photoswitching, we synchronously obtained the fluorescence spectra and positions of ∼10(6) single molecules in labeled cells in minutes, which consequently enabled spectrally resolved, 'true-color' super-resolution microscopy. The method, called spectrally resolved stochastic optical reconstruction microscopy (SR-STORM), achieved cross-talk-free three-dimensional (3D) imaging for four dyes 10 nm apart in emission spectrum. Excellent resolution was obtained for every channel, and 3D localizations of all molecules were automatically aligned within one imaging path.
Keyphrases
- single molecule
- high resolution
- atomic force microscopy
- living cells
- induced apoptosis
- optical coherence tomography
- high speed
- photodynamic therapy
- magnetic resonance imaging
- endoplasmic reticulum stress
- signaling pathway
- density functional theory
- quality improvement
- computed tomography
- pet imaging
- magnetic resonance
- cell proliferation
- molecular dynamics
- quantum dots