Potential mechanisms underlying the association between type II diabetes mellitus and cognitive dysfunction in rats: a link between miRNA-21 and Resveratrol's neuroprotective action.
Norhan S El-SayedSoha ElatrebiRasha SaidHeba F IbrahimEman M OmarPublished in: Metabolic brain disease (2022)
Cognitive impairment is considered as a typical feature of neurodegenerative diseases in diabetes mellitus (DM). However, the exact link between cognitive dysfunction and diabetes mellitus is still vague. This study aims to investigate some of the mechanisms underlying cognitive impairment that associates diabetes mellitus and insulin resistance. We investigated the role of resveratrol as well on cognitive function in experimentally induced type 2 diabetes highlighting on its influence on the expression of brain miRNA 21. Resveratrol is a naturally occurring, biologically active compound that has numerous significant impacts on the body. Type 2 diabetes mellitus was induced by high fat diet followed a single dose of streptozotocin. Diabetic rats were treated with resveratrol for four weeks. Rats were sacrificed after neurobehavioral testing. Hippocampal tissues were used to assess expression of miRNA 21, GSK and oxidative stress markers. Serum samples were obtained to determine glucose levels, lipid profile and insulin levels. Hippocampal and serum AGEs were measured as well and HOMA IR was calculated. We detected memory impairment and disturbed insulin signaling in diabetic rats. These derangements were reversed by resveratrol treatment partially due to increased expression of miRNA-21. Our study pins the role of miRNA-21 in modulating brain insulin signaling and hence alleviating cognitive dysfunction accompanying diabetes mellitus.
Keyphrases
- diabetic rats
- glycemic control
- oxidative stress
- type diabetes
- insulin resistance
- high fat diet
- blood glucose
- cognitive impairment
- poor prognosis
- adipose tissue
- cerebral ischemia
- weight loss
- machine learning
- polycystic ovary syndrome
- metabolic syndrome
- ischemia reperfusion injury
- dna damage
- white matter
- cardiovascular disease
- resting state
- induced apoptosis
- functional connectivity
- heat shock
- multiple sclerosis
- endoplasmic reticulum stress
- binding protein
- deep learning
- long non coding rna
- subarachnoid hemorrhage
- risk assessment
- cell proliferation
- blood brain barrier
- density functional theory
- high fat diet induced