Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves.
Monica DettinAnnj ZamunerFilippo NasoAntonella MonteleoneMichele SpinaGino GerosaPublished in: BioMed research international (2017)
The increasing urgency for replacement of pathological heart valves is a major stimulus for research on alternatives to glutaraldehyde-treated grafts. New xenogeneic acellular heart valve substitutes that can be repopulated by host cells are currently under investigation. Anionic surfactants, including bile acids, have been widely used to eliminate the resident cell components chiefly responsible for the immunogenicity of the tissue, even if detergent toxicity might present limitations to the survival and/or functional expression of the repopulating cells. To date, the determination of residual detergent has been carried out almost exclusively on the washings following cell removal procedures. Here, a novel HPLC-based procedure is proposed for the direct quantification of detergent (cholate, deoxycholate, and taurodeoxycholate) residues entrapped in the scaffold of decellularized porcine aortic and pulmonary valves. The method was demonstrated to be sensitive, reproducible, and extendable to different types of detergent. This assessment also revealed that cell-depleted heart valve scaffolds prepared according to procedures currently considered for clinical use might contain significant amount of surfactant.
Keyphrases
- aortic valve
- single cell
- tissue engineering
- heart failure
- induced apoptosis
- cell therapy
- aortic valve replacement
- aortic stenosis
- solid phase extraction
- poor prognosis
- transcatheter aortic valve replacement
- pulmonary hypertension
- transcatheter aortic valve implantation
- oxidative stress
- coronary artery disease
- left ventricular
- extracellular matrix
- high resolution
- minimally invasive
- long non coding rna
- signaling pathway
- bone marrow
- quality improvement
- binding protein
- liquid chromatography
- tandem mass spectrometry