Piperine Attenuates Bmal1 -Mediated Glucose Metabolism Disorder in a Trpv1 -Dependent Manner in HepG2 Cells.
Jiayi SunBiqi TangChi-Tang HoMuwen LuPublished in: Journal of agricultural and food chemistry (2023)
Piperine (PIP), a pungent alkaloid found in black pepper, has various pharmacological effects by activating the transient receptor potential vanilloid 1 (TRPV1) receptor. In this study, the regulating effect of PIP on glucose metabolism and the underlying mechanism were examined using an insulin-resistant cell model. Results showed that PIP alleviated glucosamine (GlcN)-induced glucose metabolism disorder (from 59.19 ± 1.90 to 88.36 ± 6.57%), restored cellular redox balance (from 148.43 ± 3.52 to 110.47 ± 3.52%), improved mitochondrial function (from 63.76 ± 4.87 to 85.98 ± 5.12%), and mitigated circadian disruption in HepG2 cells via the mediation of circadian clock gene Bmal1 . After the knockdown of the Trpv1 gene, the modulating effect of PIP on Bmal1 -mediated glucose metabolism was weakened, indicating that PIP alleviated Bmal1 -involved insulin resistance and circadian misalignment in a Trpv1 -dependent manner in HepG2 cells.
Keyphrases
- neuropathic pain
- insulin resistance
- type diabetes
- signaling pathway
- genome wide
- copy number
- adipose tissue
- single cell
- metabolic syndrome
- genome wide identification
- high glucose
- high fat diet
- spinal cord injury
- stem cells
- skeletal muscle
- cell therapy
- spinal cord
- glycemic control
- brain injury
- binding protein
- risk assessment
- oxidative stress
- polycystic ovary syndrome
- endothelial cells
- subarachnoid hemorrhage
- cerebral ischemia
- stress induced