The role of insulin resistance in experimental diabetic retinopathy-Genetic and molecular aspects.
Patrick JärgenAxel DietrichAndreas W HerlingHans-Peter HammesPaulus WohlfartPublished in: PloS one (2017)
The most complex genetic regulation in the retina was observed in ZDF rats with a strong overlap to STZ-Wistar rats. Surprisingly, systemic insulin resistance alone in ZF rats without concomitant hyperglycemia did not induce any significant change in retinal gene expression pattern. Pathway analysis indicate an overlap between ZDF rats and STZ-treated rats in pathways like complement system activation, acute phase response signalling, and oncostatin-M signalling. Major array gene expression changes could be confirmed by subsequent PCR. An analysis of upstream transcriptional regulators revealed interferon-γ, interleukin-6 and oncostatin-M in STZ and ZDF rats. CONCLUSIONS: Systemic hyperinsulinaemia without hyperglycemia does not result in significant gene expression changes in retina. In contrast, persistent systemic hyperglycemia boosts much stronger expression changes with a limited number of known and new key regulators.
Keyphrases
- gene expression
- diabetic retinopathy
- diabetic rats
- dna methylation
- insulin resistance
- optical coherence tomography
- type diabetes
- transcription factor
- genome wide
- oxidative stress
- metabolic syndrome
- poor prognosis
- dendritic cells
- high resolution
- computed tomography
- high throughput
- drug induced
- polycystic ovary syndrome
- heat shock protein
- contrast enhanced
- heat shock