Optimizing Human Bile Preparation for Two-Dimensional Gel Electrophoresis.
Hao-Tsai ChengSen-Yung HsiehChang-Mu SungBetty Chien-Jung PaiNai-Jen LiuCarl P C ChenPublished in: BioMed research international (2016)
Protein samples of bile prepared using acetone precipitation followed by 2D Clean-Up kit exhibited high protein resolution and significant protein profile. This optimized protein preparation protocol can effectively concentrate bile proteins, remove abundant proteins and debris, and yield clear presentation of nonabundant proteins and its isoforms on 2-dimensional electrophoresis gel images.