Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate.
Beomsue KimMasahiro FukudaJung-Yeol LeeDongdong SuSrikanta SanuAymeric SilvinAudrey T T KhooTaejoon KwonXiao LiuWeijie ChiXiaogang LiuSejong ChoiDiana S Y WanSung-Jin ParkJin-Soo KimFlorent GinhouxH Shawn JeYoung Tae ChangPublished in: Angewandte Chemie (International ed. in English) (2019)
Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure-activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.
Keyphrases
- inflammatory response
- neuropathic pain
- crispr cas
- high resolution
- spinal cord injury
- living cells
- adipose tissue
- high throughput
- resting state
- gene expression
- quality improvement
- genome editing
- sensitive detection
- patient safety
- fluorescent probe
- mass spectrometry
- fluorescence imaging
- photodynamic therapy
- quantum dots
- wild type