Differential gene expression in skin RNA of horses affected with degenerative suspensory ligament desmitis.
Abigail HaythornMadeline YoungJames StantonJian ZhangP O E MuellerJaroslava HalperPublished in: Journal of orthopaedic surgery and research (2020)
The overexpression of BMP2 correlates well with our previous data. However, the decrease in expression of numerous proteoglycans was unexpected. A mutation in a gene of a less characterized proteoglycan and/or glycosyltransferase with subsequent increased production of hyaluronan and/or a proteoglycan(s) undetected in our study could account for the systemic proteoglycan deposition. Decreased collagen gene expression indicates abnormal connective tissue metabolism. The increased expression of keratin genes and FGF5 supports reports of skin abnormalities in DSLD. Underexpression of immune function genes corresponds with lack of inflammation in DSLD tissues. Finally, though the proteoglycan and/or glycosaminoglycan abundant in DSLD has not been identified, we validated our previous data, including overexpression of BMP2, and systemic nature of DSLD due to disturbed metabolism of the extracellular matrix.
Keyphrases
- gene expression
- extracellular matrix
- genome wide
- poor prognosis
- dna methylation
- genome wide identification
- wound healing
- electronic health record
- cell proliferation
- mesenchymal stem cells
- transcription factor
- big data
- soft tissue
- oxidative stress
- copy number
- genome wide analysis
- bioinformatics analysis
- long non coding rna
- binding protein
- bone regeneration
- adverse drug
- artificial intelligence
- drug induced
- data analysis
- bone marrow