Amniotic Membrane Enhance the Effect of Vascular Endothelial Growth Factor on the Angiogenic Marker Expression of Stem Cells from Human Exfoliated Deciduous Teeth.
Muhammad Fuad Hilmi YusofSiti Nurnasihah Md HashimWafa' ZahariHamshawagini ChandraKhairul Bariah Ahmad Amin NoordinThirumulu Ponnuraj KannanSuzina Sheikh Abdul HamidKhairani Idah MokhtarAzlina AhmadPublished in: Applied biochemistry and biotechnology (2020)
Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 24-h VEGF induced on SHED endothelial differentiation when seeded on acellular stromal side (SS) of AM matrix. Stemness of SHED was identified by flow cytometry. Cell attachment and morphological changes towards the matrix was observed by scanning electron microscopy. Protein expression of endothelial marker was examined by Western blot. The expression of stem cells and endothelial-specific gene markers of VEGF-induced SHED cultured on human AM was inspected via reverse transcriptase-polymerase chain reaction. Results showed SHED at both passages retain stemness property. Ang-1 protein was expressed in SHED. Cells treated with VEGF and cultured on AM transformed attached well to AM. VEGF-induced SHED expressed both stem cell and endothelial-specific markers throughout the treatments and timeline. Interestingly, prolonged VEGF treatment increased the expression of Cox-2 and VE-Cadherin genes in all treated groups when compared to SHED. It was concluded that the VEGF-induced SHED showed better expression of endothelial-specific markers when cultured on SS of AM, with prolonged VEGF treatment.
Keyphrases
- endothelial cells
- high glucose
- vascular endothelial growth factor
- stem cells
- poor prognosis
- diabetic rats
- flow cytometry
- electron microscopy
- binding protein
- epithelial mesenchymal transition
- cell therapy
- genome wide
- bone marrow
- drug induced
- dna methylation
- cell proliferation
- gene expression
- south africa
- mesenchymal stem cells
- transcription factor
- endoplasmic reticulum stress
- small molecule
- mass spectrometry
- newly diagnosed
- single cell
- angiotensin ii
- cancer stem cells