Immunohistochemical examination of osteopontin and sirtuin-1 expression in cattle tuberculosis.
M SevtekinOzlem OzmenPublished in: Biotechnic & histochemistry : official publication of the Biological Stain Commission (2018)
We evaluated osteopontin (OPN) and sirtuin-1 (SIRT-1) expression in bovine tuberculosis lesions. The organs of cattle with tuberculosis (TB) were examined for morphology, histopathology and immunohistochemistry of OPN and SIRT-1 expression. Macroscopic lesions commonly were localized in the lungs and mediastinal lymph nodes as well as in livers and hearts. Mycobacterial agents were detected in lesions using the Ziehl-Neelsen method. No OPN or SIRT-1 expression was detected by immunohistochemistry in normal tissues, whereas a marked increase in their expressions was observed in tuberculous lesions. The most intense immunopositive cells were Langerhans giant cells and inflammatory cells. Our findings indicate that OPN and SIRT-1 participate in the pathogenesis of bovine TB.
Keyphrases
- mycobacterium tuberculosis
- induced apoptosis
- poor prognosis
- oxidative stress
- lymph node
- cell cycle arrest
- binding protein
- ischemia reperfusion injury
- endoplasmic reticulum stress
- emergency department
- pulmonary tuberculosis
- long non coding rna
- hiv aids
- cell death
- human immunodeficiency virus
- pi k akt
- adverse drug
- cell proliferation
- neoadjuvant chemotherapy
- hiv infected