Green biomimetic silver nanoparticles utilizing the red algae Amphiroa rigida and its potent antibacterial, cytotoxicity and larvicidal efficiency.
Mani GopuPonnuchamy KumarThangasamy SelvankumarBalakrishnan SenthilkumarChinnappan SudhakarMuthusamy GovarthananRathakrishnan Selva KumarKandasamy SelvamPublished in: Bioprocess and biosystems engineering (2020)
The present investigation reports a simple, rapid, inexpensive, and eco-friendly approach for synthesizing Amphiroa rigida-mediated silver nanoparticles (AR-AgNPs) for the first time. The biomimetic synthesized AR-AgNPs were characterized by both spectral and microscopic analysis. The UV-visible spectrum showed the surface plasmon peak at 420 nm, which indicated the formation of AR-AgNPs. X-ray diffraction characterization of AR-AgNPs showed a face-centered cubic crystal (25 nm) and the transmission electron microscope micrograph showed spherical shape. The functional group of polysaccharide that plays a major role as a stabilizing and reducing agent is confirmed by Fourier-transform infrared spectroscopy. The biomimetic synthesized AR-AgNPs showed antibacterial activity against Staphylococcus aureus (21 ± 0.2 mm) and Pseudomonas aeruginosa (15 ± 0.2 mm). Further, the cytotoxic effects of AR-AgNPs against MCF-7 human breast cancer cells were observed through acridine orange-ethidium bromide and Hoechst staining. Besides, AR-AgNPs are found to be inhibit the growth of 3rd and 4th instar larvae of Aedes aegypti in a dose-dependent manner.
Keyphrases
- silver nanoparticles
- aedes aegypti
- breast cancer cells
- pseudomonas aeruginosa
- staphylococcus aureus
- endothelial cells
- zika virus
- cystic fibrosis
- high resolution
- magnetic resonance imaging
- magnetic resonance
- biofilm formation
- mass spectrometry
- adverse drug
- crystal structure
- loop mediated isothermal amplification
- pluripotent stem cells
- candida albicans