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The long noncoding RNA Morrbid regulates CD8 T cells in response to viral infection.

Jonathan J KotzinFany IsekaJasmine WrightMegha G BasavappaMegan L ClarkMohammed-Alkhatim AliMohamed S Abdel HakeemTanner F RobertsonWalter K MowelLeonel JoannasVanessa D NealSean P SpencerCamille M SyrettMontserrat C AngueraAdam WilliamsE John WherryJorge Henao-Mejia
Published in: Proceedings of the National Academy of Sciences of the United States of America (2019)
The transcriptional programs that regulate CD8 T-cell differentiation and function in the context of viral infections or tumor immune surveillance have been extensively studied; yet how long noncoding RNAs (lncRNAs) and the loci that transcribe them contribute to the regulation of CD8 T cells during viral infections remains largely unexplored. Here, we report that transcription of the lncRNA Morrbid is specifically induced by T-cell receptor (TCR) and type I IFN stimulation during the early stages of acute and chronic lymphocytic choriomeningitis virus (LCMV) infection. In response to type I IFN, the Morrbid RNA and its locus control CD8 T cell expansion, survival, and effector function by regulating the expression of the proapoptotic factor, Bcl2l11, and by modulating the strength of the PI3K-AKT signaling pathway. Thus, our results demonstrate that inflammatory cue-responsive lncRNA loci represent fundamental mechanisms by which CD8 T cells are regulated in response to pathogens and potentially cancer.
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