Anopheles gambiae mosGILT regulates innate immune genes and zpg expression.
Gunjan AroraXiaotian TangYingjun CuiJing YangYu-Min ChuangJayadev JoshiAndaleeb SajidYuemei DongPeter CresswellGeorge DimopoulosErol FikrigPublished in: bioRxiv : the preprint server for biology (2023)
Gene-edited mosquitoes lacking a g amma-interferon-inducible lysosomal thiol reductase-like protein, namely ( mosGILT null ) have lower Plasmodium infection, which is linked to impaired ovarian development and immune activation. The transcriptome of mosGILT null A. gambiae was therefore compared to wild type (WT) by RNA-sequencing to delineate mosGILT-dependent pathways. Compared to WT mosquitoes, mosGILT null A. gambiae demonstrated altered expression of genes related to oogenesis, 20-hydroxyecdysone synthesis, as well as immune-related genes. Serendipitously, the zero population growth gene, zpg , an essential regulator of germ cell development was found to be one of the most downregulated genes in mosGILT null mosquitoes. These results provide the crucial missing link between two previous studies on the role of zpg and mosGILT in ovarian development. This study further demonstrates that mosGILT has the potential to serve as a target for the biological control of mosquito vectors and to influence the Plasmodium life cycle within the vector.
Keyphrases
- aedes aegypti
- genome wide
- genome wide identification
- dengue virus
- poor prognosis
- zika virus
- innate immune
- life cycle
- wild type
- germ cell
- copy number
- transcription factor
- genome wide analysis
- dna methylation
- single cell
- bioinformatics analysis
- crispr cas
- gene expression
- immune response
- binding protein
- rna seq
- risk assessment
- plasmodium falciparum
- gene therapy
- drug induced