Combined IL-2, agonistic CD3 and 4-1BB stimulation preserve clonotype hierarchy in propagated non-small cell lung cancer tumor-infiltrating lymphocytes.
Parin ShahMarie-Andrée ForgetMeredith L FrankPeixin JiangDonastas Sakellariou-ThompsonLorenzo FedericoRoohussaba KhairullahChantal Alexia NeutzlerIgnacio WistubaChi-Wan B ChowYan LongJunya FujimotoShiaw-Yih LinAnirban MaitraMarcelo Vailati NegrãoKyle Gregory MitchellAnnikka WeissferdtAra A VaporciyanTina CasconeJack A RothJianjun ZhangBoris SepesiDon L GibbonsJohn V HeymachCara L HaymakerDaniel J McGrailAlexandre ReubenChantale BernatchezPublished in: Journal for immunotherapy of cancer (2022)
We report the feasibility of robustly expanding a T-cell repertoire recapitulating the clonal hierarchy of the T cells in the NSCLC tumor, including a large number of putative tumor-specific TIL clones, using the TIL 3.0 methodology. If scaled up and employed as a sole expansion platform, the robustness and speed of TIL 3.0 may facilitate the testing of TIL-ACT approaches in NSCLC.